DBNascent | Samples

SRZ7184282 Sample ID 2849

**Data sources**
Paper identifier	Zhao2019myod
SRP accession	SRP148464
GEO accession	GSE114657
Samples	SRR7184282
	SRR7184283

**Technical details**
QC score	2
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	112 Mb
Stranded gene read counts	TXT file	927 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	5 Mb
dREG bidirectional regions	BED file	2 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	C2C12
Clone/Individual
Strain
Genotype	MyoD -/-
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	myotube differentiation protocol	-48 hr	0 hr	2 day

**Extended notes**
Pipeline info and software versions
Sample notes	Labeled GRO-seq, but they used 'biotin-11-UTP'
Analysis notes

**Sample metrics**
Sample QC score	2
Sample NRO score	3
Raw read length	75
Read depth after trimming	53,167,671
Duplication proportion	0.59825
Proportion reads mapped	0.8651
Exon/intron ratio	6.27903
Unique read proportion	0.49907

**Bidirectionals**
Total # Tfit bidirectionals	54263
# Promoter Tfit bidirectionals	13027
# Intronic Tfit bidirectionals	20320
# Intergenic Tfit bidirectionals	18110
Total # dREG bidirectionals	43230
# Promoter dREG bidirectionals	7211
# Intronic dREG bidirectionals	18001
# Intergenic dREG bidirectionals	14642
Part of Tfit master merge?	True
Part of dREG master merge?	True