DBNascent | Samples

SRZ7746877 Sample ID 2771

**Data sources**
Paper identifier	Zhang2018timing
SRP accession	SRP158674
GEO accession	GSE118957
Samples	SRR7746877
	SRR7746878
	SRR7746879

**Technical details**
QC score	4
Replicate	2
Protocol	PRO-seq variant
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	47 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	775 Kb
dREG bidirectional regions	BED file	337 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	time variant PRO-seq; 2 min run-on time
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	2
Raw read length	51
Read depth after trimming	52,175,749
Duplication proportion	0.90166
Proportion reads mapped	0.9012
Exon/intron ratio	3.69553
Unique read proportion	0.17165

**Bidirectionals**
Total # Tfit bidirectionals	8780
# Promoter Tfit bidirectionals	3523
# Intronic Tfit bidirectionals	3519
# Intergenic Tfit bidirectionals	969
Total # dREG bidirectionals	7865
# Promoter dREG bidirectionals	5482
# Intronic dREG bidirectionals	2089
# Intergenic dREG bidirectionals	717
Part of Tfit master merge?	True
Part of dREG master merge?	True