DBNascent | Samples

SRZ7879154 Sample ID 2280

**Data sources**
Paper identifier	Sheridan2019widespread
SRP accession	SRP162163
GEO accession	GSE120201
Samples	SRR7879154
	SRR7879155

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	32 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	327 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T Flp-In
Clone/Individual
Strain
Genotype	dox-inducible TFIIS D282A, E283A (Flp knock-in)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	doxycycline (2 microg/mL)	-1 day	0 day	1 day

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	2
Raw read length	51
Read depth after trimming	21,603,771
Duplication proportion	0.87191
Proportion reads mapped	0.8998
Exon/intron ratio	4.11187
Unique read proportion	0.23202

**Bidirectionals**
Total # Tfit bidirectionals	20256
# Promoter Tfit bidirectionals	7925
# Intronic Tfit bidirectionals	9412
# Intergenic Tfit bidirectionals	1591
Total # dREG bidirectionals	7600
# Promoter dREG bidirectionals	4069
# Intronic dREG bidirectionals	2533
# Intergenic dREG bidirectionals	799
Part of Tfit master merge?	True
Part of dREG master merge?	True