DBNascent | Samples

SRZ826217 Sample ID 1422

**Data sources**
Paper identifier	Lam2013rev
SRP accession	SRP021025
GEO accession	GSE45914
Samples	SRR826217
	SRR826218
	SRR826219
	SRR826220
	SRR826221
	SRR826222
	SRR826223
	SRR826224
	SRR868693

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	173 Mb
Stranded gene read counts	TXT file	934 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	bone marrow derived macrophage
Clone/Individual
Strain	C57BL/6
Genotype	Rev-erb-alpha fl/fl; Rev-erb-beta fl/fl; Tie2-Cre
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Preseq required separate run with -D flag

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	1
Read depth after trimming	185,732,020
Duplication proportion	0.86157
Proportion reads mapped	0.6543
Exon/intron ratio	2.73525
Unique read proportion	0.50218

**Bidirectionals**
Total # Tfit bidirectionals	30148
# Promoter Tfit bidirectionals	10047
# Intronic Tfit bidirectionals	10153
# Intergenic Tfit bidirectionals	7006
Total # dREG bidirectionals	24219
# Promoter dREG bidirectionals	6574
# Intronic dREG bidirectionals	9200
# Intergenic dREG bidirectionals	6445
Part of Tfit master merge?	False
Part of dREG master merge?	False