DBNascent | Samples

SRZ8283305 Sample ID 2749

**Data sources**
Paper identifier	Yu2020negative
SRP accession	SRP172558
GEO accession	GSE123370
Samples	SRR8283305
	SRR8283306

**Technical details**
QC score	2
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 forward
Reverse complemented?	True
Mapped strandedness	read1 forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	54 Mb
Stranded gene read counts	TXT file	913 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	718 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	bone marrow derived macrophage
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	lipopolysaccharide (10 ng/mL)	-30 min	0 min	30 min

**Extended notes**
Pipeline info and software versions
Sample notes	Replicates not explicitly stated
Analysis notes

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	150
Read depth after trimming	23,183,659
Duplication proportion	0.46668
Proportion reads mapped	0.7291
Exon/intron ratio	4.57859
Unique read proportion	0.47797

**Bidirectionals**
Total # Tfit bidirectionals	22257
# Promoter Tfit bidirectionals	8023
# Intronic Tfit bidirectionals	7710
# Intergenic Tfit bidirectionals	3760
Total # dREG bidirectionals	16546
# Promoter dREG bidirectionals	5185
# Intronic dREG bidirectionals	5640
# Intergenic dREG bidirectionals	3210
Part of Tfit master merge?	True
Part of dREG master merge?	True