DBNascent | Samples

SRZ8283309 Sample ID 2751

**Data sources**
Paper identifier	Yu2020negative
SRP accession	SRP172558
GEO accession	GSE123370
Samples	SRR8283309
	SRR8283310

**Technical details**
QC score	2
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 forward
Reverse complemented?	True
Mapped strandedness	read1 forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	60 Mb
Stranded gene read counts	TXT file	914 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	765 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	bone marrow derived macrophage
Clone/Individual
Strain
Genotype	NelfB -/-
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	lipopolysaccharide (10 ng/mL)	-30 min	0 min	30 min

**Extended notes**
Pipeline info and software versions
Sample notes	Replicates not explicitly stated
Analysis notes

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	150
Read depth after trimming	26,239,388
Duplication proportion	0.46833
Proportion reads mapped	0.7302
Exon/intron ratio	4.57291
Unique read proportion	0.49093

**Bidirectionals**
Total # Tfit bidirectionals	23225
# Promoter Tfit bidirectionals	8324
# Intronic Tfit bidirectionals	8009
# Intergenic Tfit bidirectionals	4062
Total # dREG bidirectionals	17610
# Promoter dREG bidirectionals	5343
# Intronic dREG bidirectionals	6148
# Intergenic dREG bidirectionals	3531
Part of Tfit master merge?	True
Part of dREG master merge?	True