DBNascent | Samples

SRZ8795375 Sample ID 1266

**Data sources**
Paper identifier	Kriaucionis2019unpublished
SRP accession	SRP189657
GEO accession	GSE128951
Samples	SRR8795375
	SRR8795376
	SRR8795377
	SRR8795378
	SRR8795379
	SRR8795380
	SRR8795381
	SRR8795382

**Technical details**
QC score	4
Replicate	3
Protocol	PRO-cap
Library prep	ligation
Spike-in	G. gallus
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	44 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T Flp-In
Clone/Individual
Strain
Genotype
Construct	dox-inducible Tet-CD-GFP

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Not clear what construct is or whether it's genomically integrated, but it may be the catalytic domain of Tet1 or another human Tet protein (not published or detailed in GEO)
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	5
Raw read length	35
Read depth after trimming	40,646,990
Duplication proportion	0.81551
Proportion reads mapped	0.5297
Exon/intron ratio	17.5261
Unique read proportion	0.31193

**Bidirectionals**
Total # Tfit bidirectionals	30270
# Promoter Tfit bidirectionals	13778
# Intronic Tfit bidirectionals	11562
# Intergenic Tfit bidirectionals	6093
Total # dREG bidirectionals	25328
# Promoter dREG bidirectionals	10814
# Intronic dREG bidirectionals	9854
# Intergenic dREG bidirectionals	4382
Part of Tfit master merge?	False
Part of dREG master merge?	False