DBNascent | Samples

SRZ8795383 Sample ID 1267

**Data sources**
Paper identifier	Kriaucionis2019unpublished
SRP accession	SRP189657
GEO accession	GSE128951
Samples	SRR8795383
	SRR8795384
	SRR8795385
	SRR8795386
	SRR8795387
	SRR8795388
	SRR8795389
	SRR8795390

**Technical details**
QC score	4
Replicate	1
Protocol	PRO-cap
Library prep	ligation
Spike-in	G. gallus
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	42 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T Flp-In
Clone/Individual
Strain
Genotype
Construct	dox-inducible Tet-CD-GFP

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	5-aza-2'-deoxycytidine (880 nM)	-2 day	0 day	2 day

**Extended notes**
Pipeline info and software versions
Sample notes	Not clear what construct is or whether it's genomically integrated, but it may be the catalytic domain of Tet1 or another human Tet protein (not published or detailed in GEO)
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	5
Raw read length	35
Read depth after trimming	36,434,080
Duplication proportion	0.80037
Proportion reads mapped	0.5159
Exon/intron ratio	16.8467
Unique read proportion	0.31559

**Bidirectionals**
Total # Tfit bidirectionals	30288
# Promoter Tfit bidirectionals	14092
# Intronic Tfit bidirectionals	11618
# Intergenic Tfit bidirectionals	5708
Total # dREG bidirectionals	24733
# Promoter dREG bidirectionals	11049
# Intronic dREG bidirectionals	9693
# Intergenic dREG bidirectionals	3899
Part of Tfit master merge?	False
Part of dREG master merge?	False