DBNascent | Samples

SRZ8795391 Sample ID 1268

**Data sources**
Paper identifier	Kriaucionis2019unpublished
SRP accession	SRP189657
GEO accession	GSE128951
Samples	SRR8795391
	SRR8795392
	SRR8795393
	SRR8795394
	SRR8795395
	SRR8795396
	SRR8795397
	SRR8795398

**Technical details**
QC score	4
Replicate	2
Protocol	PRO-cap
Library prep	ligation
Spike-in	G. gallus
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	45 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T Flp-In
Clone/Individual
Strain
Genotype
Construct	dox-inducible Tet-CD-GFP

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	5-aza-2'-deoxycytidine (880 nM)	-2 day	0 day	2 day

**Extended notes**
Pipeline info and software versions
Sample notes	Not clear what construct is or whether it's genomically integrated, but it may be the catalytic domain of Tet1 or another human Tet protein (not published or detailed in GEO)
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	5
Raw read length	35
Read depth after trimming	38,438,107
Duplication proportion	0.81027
Proportion reads mapped	0.5605
Exon/intron ratio	17.6562
Unique read proportion	0.31584

**Bidirectionals**
Total # Tfit bidirectionals	31011
# Promoter Tfit bidirectionals	14074
# Intronic Tfit bidirectionals	12001
# Intergenic Tfit bidirectionals	6055
Total # dREG bidirectionals	25468
# Promoter dREG bidirectionals	10852
# Intronic dREG bidirectionals	10136
# Intergenic dREG bidirectionals	4262
Part of Tfit master merge?	False
Part of dREG master merge?	False