SRZ8795399 Sample ID 1269

Data sources
Paper identifier Kriaucionis2019unpublished
SRP accession SRP189657
GEO accession GSE128951
Samples SRR8795399
SRR8795400
SRR8795401
SRR8795402
SRR8795403
SRR8795404
SRR8795405
SRR8795406
Technical details
QC score 4
Replicate 3
Protocol PRO-cap
Library prep ligation
Spike-in G. gallus
Single/Paired end single
Raw strandedness forward
Reverse complemented? False
Mapped strandedness forward
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file 48 Mb
Stranded gene read counts TXT file 1 Mb
Unstranded bidirectional read counts TXT file 21 Mb
Tfit bidirectional regions BED file 3 Mb
dREG bidirectional regions BED file 1 Mb
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type HEK293T Flp-In
Clone/Individual
Strain
Genotype
Construct dox-inducible Tet-CD-GFP
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment 5-aza-2'-deoxycytidine (880 nM) -2 day 0 day 2 day
Extended notes
Pipeline info and software versions
Sample notes Not clear what construct is or whether it's genomically integrated, but it may be the catalytic domain of Tet1 or another human Tet protein (not published or detailed in GEO)
Analysis notes Ran qc redo nextflow
Sample metrics
Sample QC score 4
Sample NRO score 5
Raw read length 35
Read depth after trimming 39,962,879
Duplication proportion 0.81317
Proportion reads mapped 0.5906
Exon/intron ratio 19.1327
Unique read proportion 0.31719
Bidirectionals
Total # Tfit bidirectionals 35557
# Promoter Tfit bidirectionals 13669
# Intronic Tfit bidirectionals 13654
# Intergenic Tfit bidirectionals 9043
Total # dREG bidirectionals 28345
# Promoter dREG bidirectionals 10684
# Intronic dREG bidirectionals 11007
# Intergenic dREG bidirectionals 6036
Part of Tfit master merge? False
Part of dREG master merge? False