DBNascent | Samples

SRZ8795407 Sample ID 1270

**Data sources**
Paper identifier	Kriaucionis2019unpublished
SRP accession	SRP189657
GEO accession	GSE128951
Samples	SRR8795407
	SRR8795408
	SRR8795409
	SRR8795410
	SRR8795411
	SRR8795412
	SRR8795413
	SRR8795414

**Technical details**
QC score	4
Replicate	1
Protocol	PRO-cap
Library prep	ligation
Spike-in	G. gallus
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	38 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T Flp-In
Clone/Individual
Strain
Genotype
Construct	dox-inducible Tet-CD-GFP

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	doxycycline (1 microg/mL)	-2 day	0 day	2 day

**Extended notes**
Pipeline info and software versions
Sample notes	Not clear what construct is or whether it's genomically integrated, but it may be the catalytic domain of Tet1 or another human Tet protein (not published or detailed in GEO)
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	5
Raw read length	35
Read depth after trimming	37,653,210
Duplication proportion	0.81104
Proportion reads mapped	0.4832
Exon/intron ratio	16.6008
Unique read proportion	0.30074

**Bidirectionals**
Total # Tfit bidirectionals	29069
# Promoter Tfit bidirectionals	14311
# Intronic Tfit bidirectionals	10899
# Intergenic Tfit bidirectionals	5145
Total # dREG bidirectionals	23910
# Promoter dREG bidirectionals	11148
# Intronic dREG bidirectionals	9185
# Intergenic dREG bidirectionals	3594
Part of Tfit master merge?	False
Part of dREG master merge?	False