DBNascent | Samples

SRZ8795431 Sample ID 1273

**Data sources**
Paper identifier	Kriaucionis2019unpublished
SRP accession	SRP189657
GEO accession	GSE128951
Samples	SRR8795431
	SRR8795432
	SRR8795433
	SRR8795434
	SRR8795435
	SRR8795436
	SRR8795437
	SRR8795438

**Technical details**
QC score	1
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	G. gallus
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	106 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	699 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T Flp-In
Clone/Individual
Strain
Genotype
Construct	dox-inducible Tet-CD-GFP

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Not clear what construct is or whether it's genomically integrated, but it may be the catalytic domain of Tet1 or another human Tet protein (not published or detailed in GEO)
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	35
Read depth after trimming	83,749,329
Duplication proportion	0.31441
Proportion reads mapped	0.278
Exon/intron ratio	4.29211
Unique read proportion	0.76038

**Bidirectionals**
Total # Tfit bidirectionals	27160
# Promoter Tfit bidirectionals	15128
# Intronic Tfit bidirectionals	9927
# Intergenic Tfit bidirectionals	2678
Total # dREG bidirectionals	15859
# Promoter dREG bidirectionals	7500
# Intronic dREG bidirectionals	5797
# Intergenic dREG bidirectionals	1470
Part of Tfit master merge?	False
Part of dREG master merge?	False