DBNascent | Samples

SRZ8795439 Sample ID 1274

**Data sources**
Paper identifier	Kriaucionis2019unpublished
SRP accession	SRP189657
GEO accession	GSE128951
Samples	SRR8795439
	SRR8795440
	SRR8795441
	SRR8795442
	SRR8795443
	SRR8795444
	SRR8795445
	SRR8795446

**Technical details**
QC score	1
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	G. gallus
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	133 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	789 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T Flp-In
Clone/Individual
Strain
Genotype
Construct	dox-inducible Tet-CD-GFP

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Not clear what construct is or whether it's genomically integrated, but it may be the catalytic domain of Tet1 or another human Tet protein (not published or detailed in GEO)
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	35
Read depth after trimming	86,515,265
Duplication proportion	0.31247
Proportion reads mapped	0.3383
Exon/intron ratio	4.5319
Unique read proportion	0.79683

**Bidirectionals**
Total # Tfit bidirectionals	29412
# Promoter Tfit bidirectionals	16500
# Intronic Tfit bidirectionals	10848
# Intergenic Tfit bidirectionals	3088
Total # dREG bidirectionals	17828
# Promoter dREG bidirectionals	7452
# Intronic dREG bidirectionals	7093
# Intergenic dREG bidirectionals	2000
Part of Tfit master merge?	False
Part of dREG master merge?	False