DBNascent | Samples

SRZ8795447 Sample ID 1275

**Data sources**
Paper identifier	Kriaucionis2019unpublished
SRP accession	SRP189657
GEO accession	GSE128951
Samples	SRR8795447
	SRR8795448
	SRR8795449
	SRR8795450
	SRR8795451
	SRR8795452
	SRR8795453
	SRR8795454

**Technical details**
QC score	1
Replicate	3
Protocol	PRO-seq
Library prep	ligation
Spike-in	G. gallus
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	132 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	790 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T Flp-In
Clone/Individual
Strain
Genotype
Construct	dox-inducible Tet-CD-GFP

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Not clear what construct is or whether it's genomically integrated, but it may be the catalytic domain of Tet1 or another human Tet protein (not published or detailed in GEO)
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	35
Read depth after trimming	82,826,733
Duplication proportion	0.3152
Proportion reads mapped	0.3515
Exon/intron ratio	4.49588
Unique read proportion	0.78959

**Bidirectionals**
Total # Tfit bidirectionals	31206
# Promoter Tfit bidirectionals	16467
# Intronic Tfit bidirectionals	11320
# Intergenic Tfit bidirectionals	3719
Total # dREG bidirectionals	17819
# Promoter dREG bidirectionals	7268
# Intronic dREG bidirectionals	6821
# Intergenic dREG bidirectionals	2214
Part of Tfit master merge?	False
Part of dREG master merge?	False