DBNascent | Samples

SRZ8795455 Sample ID 1276

**Data sources**
Paper identifier	Kriaucionis2019unpublished
SRP accession	SRP189657
GEO accession	GSE128951
Samples	SRR8795455
	SRR8795456
	SRR8795457
	SRR8795458
	SRR8795459
	SRR8795460
	SRR8795461
	SRR8795462

**Technical details**
QC score	1
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	G. gallus
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	137 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	747 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T Flp-In
Clone/Individual
Strain
Genotype
Construct	dox-inducible Tet-CD-GFP

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	5-aza-2'-deoxycytidine (880 nM)	-2 day	0 day	2 day

**Extended notes**
Pipeline info and software versions
Sample notes	Not clear what construct is or whether it's genomically integrated, but it may be the catalytic domain of Tet1 or another human Tet protein (not published or detailed in GEO)
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	35
Read depth after trimming	81,860,684
Duplication proportion	0.29716
Proportion reads mapped	0.3634
Exon/intron ratio	4.25424
Unique read proportion	0.81021

**Bidirectionals**
Total # Tfit bidirectionals	26736
# Promoter Tfit bidirectionals	14769
# Intronic Tfit bidirectionals	9724
# Intergenic Tfit bidirectionals	2879
Total # dREG bidirectionals	16933
# Promoter dREG bidirectionals	7386
# Intronic dREG bidirectionals	6584
# Intergenic dREG bidirectionals	1754
Part of Tfit master merge?	False
Part of dREG master merge?	False