DBNascent | Samples

SRZ8795463 Sample ID 1277

**Data sources**
Paper identifier	Kriaucionis2019unpublished
SRP accession	SRP189657
GEO accession	GSE128951
Samples	SRR8795463
	SRR8795464
	SRR8795465
	SRR8795466
	SRR8795467
	SRR8795468
	SRR8795469
	SRR8795470

**Technical details**
QC score	1
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	G. gallus
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	133 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	759 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T Flp-In
Clone/Individual
Strain
Genotype
Construct	dox-inducible Tet-CD-GFP

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	5-aza-2'-deoxycytidine (880 nM)	-2 day	0 day	2 day

**Extended notes**
Pipeline info and software versions
Sample notes	Not clear what construct is or whether it's genomically integrated, but it may be the catalytic domain of Tet1 or another human Tet protein (not published or detailed in GEO)
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	35
Read depth after trimming	78,508,679
Duplication proportion	0.29103
Proportion reads mapped	0.362
Exon/intron ratio	4.5
Unique read proportion	0.8134

**Bidirectionals**
Total # Tfit bidirectionals	27361
# Promoter Tfit bidirectionals	15316
# Intronic Tfit bidirectionals	10057
# Intergenic Tfit bidirectionals	2850
Total # dREG bidirectionals	17205
# Promoter dREG bidirectionals	7422
# Intronic dREG bidirectionals	6559
# Intergenic dREG bidirectionals	1837
Part of Tfit master merge?	False
Part of dREG master merge?	False