DBNascent | Samples

SRZ8795479 Sample ID 1279

**Data sources**
Paper identifier	Kriaucionis2019unpublished
SRP accession	SRP189657
GEO accession	GSE128951
Samples	SRR8795479
	SRR8795480
	SRR8795481
	SRR8795482
	SRR8795483
	SRR8795484
	SRR8795485
	SRR8795486

**Technical details**
QC score	1
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	G. gallus
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	130 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	722 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T Flp-In
Clone/Individual
Strain
Genotype
Construct	dox-inducible Tet-CD-GFP

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	doxycycline (1 microg/mL)	-2 day	0 day	2 day

**Extended notes**
Pipeline info and software versions
Sample notes	Not clear what construct is or whether it's genomically integrated, but it may be the catalytic domain of Tet1 or another human Tet protein (not published or detailed in GEO)
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	35
Read depth after trimming	82,775,659
Duplication proportion	0.30808
Proportion reads mapped	0.3509
Exon/intron ratio	4.02307
Unique read proportion	0.79183

**Bidirectionals**
Total # Tfit bidirectionals	26386
# Promoter Tfit bidirectionals	14455
# Intronic Tfit bidirectionals	9582
# Intergenic Tfit bidirectionals	2760
Total # dREG bidirectionals	16390
# Promoter dREG bidirectionals	7337
# Intronic dREG bidirectionals	6163
# Intergenic dREG bidirectionals	1645
Part of Tfit master merge?	False
Part of dREG master merge?	False