DBNascent | Samples

SRZ8795495 Sample ID 1281

**Data sources**
Paper identifier	Kriaucionis2019unpublished
SRP accession	SRP189657
GEO accession	GSE128951
Samples	SRR8795495
	SRR8795496
	SRR8795497
	SRR8795498
	SRR8795499
	SRR8795500
	SRR8795501
	SRR8795502

**Technical details**
QC score	1
Replicate	3
Protocol	PRO-seq
Library prep	ligation
Spike-in	G. gallus
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	129 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	772 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T Flp-In
Clone/Individual
Strain
Genotype
Construct	dox-inducible Tet-CD-GFP

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	doxycycline (1 microg/mL)	-2 day	0 day	2 day

**Extended notes**
Pipeline info and software versions
Sample notes	Not clear what construct is or whether it's genomically integrated, but it may be the catalytic domain of Tet1 or another human Tet protein (not published or detailed in GEO)
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	35
Read depth after trimming	78,854,001
Duplication proportion	0.3203
Proportion reads mapped	0.3653
Exon/intron ratio	4.48835
Unique read proportion	0.77915

**Bidirectionals**
Total # Tfit bidirectionals	29706
# Promoter Tfit bidirectionals	15220
# Intronic Tfit bidirectionals	10864
# Intergenic Tfit bidirectionals	3858
Total # dREG bidirectionals	17468
# Promoter dREG bidirectionals	7186
# Intronic dREG bidirectionals	6622
# Intergenic dREG bidirectionals	2190
Part of Tfit master merge?	False
Part of dREG master merge?	False