DBNascent | Samples

SRZ7349485 Sample ID 1214

**Data sources**
Paper identifier	Kloetgen2020three
SRP accession	SRP150649
GEO accession	GSE115894
Samples	SRR7349485
	SRR7349486

**Technical details**
QC score	2
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	55 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	741 Kb
dREG bidirectional regions	BED file	165 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	CUTLL1
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	gamma-secretase inhibitor (1 microM)	-73 hr	-1 hr	3 day

**Extended notes**
Pipeline info and software versions
Sample notes	gamma-secretase inhibitor is Compound E, Alexis Biochemicals
Analysis notes

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	51
Read depth after trimming	20,549,473
Duplication proportion	0.63535
Proportion reads mapped	0.9582
Exon/intron ratio	3.57407
Unique read proportion	0.41422

**Bidirectionals**
Total # Tfit bidirectionals	8490
# Promoter Tfit bidirectionals	2378
# Intronic Tfit bidirectionals	4238
# Intergenic Tfit bidirectionals	900
Total # dREG bidirectionals	3835
# Promoter dREG bidirectionals	1423
# Intronic dREG bidirectionals	1670
# Intergenic dREG bidirectionals	621
Part of Tfit master merge?	True
Part of dREG master merge?	True