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Gene read counts	Bidirectional read counts	Tfit regions	dREG regions
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Technical details

Sample name	Sample ID	Paper identifier	Protocol	Library prep	Control/Experimental	Wildtype & Untreated	QC score	Replicate
SRR8456237	2025	Parida2019nucleotide	PRO-cap	ligation	experimental	False	4	1
SRR8456238	2026	Parida2019nucleotide	PRO-cap	ligation	experimental	False	5	1
SRR8463311	2029	Parida2019nucleotide	PRO-cap	ligation	control	True	4	1
SRR8463312	2030	Parida2019nucleotide	PRO-cap	ligation	control	True	4	1
SRR7041686	2018	Parida2019nucleotide	PRO-seq	ligation	control	True	2	1
SRR7041689	2019	Parida2019nucleotide	PRO-seq	ligation	control	True	2	1
SRR7041690	2020	Parida2019nucleotide	PRO-seq	ligation	control	True	2	1
SRR8451931	2021	Parida2019nucleotide	PRO-seq	ligation	experimental	False	2	1
SRR8451932	2022	Parida2019nucleotide	PRO-seq	ligation	experimental	False	3	1
SRR8452660	2023	Parida2019nucleotide	PRO-seq	ligation	experimental	False	4	1
SRR8452661	2024	Parida2019nucleotide	PRO-seq	ligation	experimental	False	4	1
SRR8457216	2027	Parida2019nucleotide	PRO-seq	ligation	experimental	False	2	1
SRR8457217	2028	Parida2019nucleotide	PRO-seq	ligation	experimental	False	1	1

Cellular details

Sample name	Organism	Sample type	Cell type	Clone/Individual	Strain	Genotype	Construct
SRR8456237	H. sapiens	primary cell	foreskin fibroblast
SRR8456238	H. sapiens	primary cell	foreskin fibroblast
SRR8463311	H. sapiens	primary cell	foreskin fibroblast
SRR8463312	H. sapiens	primary cell	foreskin fibroblast
SRR7041686	H. sapiens	primary cell	foreskin fibroblast
SRR7041689	H. sapiens	primary cell	foreskin fibroblast
SRR7041690	H. sapiens	primary cell	foreskin fibroblast
SRR8451931	H. sapiens	primary cell	foreskin fibroblast
SRR8451932	H. sapiens	primary cell	foreskin fibroblast
SRR8452660	H. sapiens	primary cell	foreskin fibroblast
SRR8452661	H. sapiens	primary cell	foreskin fibroblast
SRR8457216	H. sapiens	primary cell	foreskin fibroblast
SRR8457217	H. sapiens	primary cell	foreskin fibroblast

Conditions

Sample name	Condition type	Treatment	Start time	End time	Duration
SRR8456237	vehicle	DMSO (0.1%)	-1 hr	0 hr	1 hr
	treatment	HCMV strain Towne varS	-5760 min	-5670 min	90 min
SRR8456238	treatment	flavopiridol (1 microM)	-1 hr	0 hr	1 hr
	treatment	HCMV strain Towne varS	-5760 min	-5670 min	90 min
SRR8463311	vehicle	DMSO (0.1%)	-1 hr	0 hr	1 hr
	vehicle	Mock infection	-5760 min	-5670 min	90 min
SRR8463312	treatment	flavopiridol (1 microM)	-1 hr	0 hr	1 hr
	vehicle	Mock infection	-5760 min	-5670 min	90 min
SRR7041686	vehicle	DMSO (0.1%)	-1 hr	0 hr	1 hr
	vehicle	Mock infection	-4 hr	0 hr	4 hr
SRR7041689	vehicle	DMSO (0.1%)	-1 hr	0 hr	1 hr
	vehicle	Mock infection	-5760 min	-5670 min	90 min
SRR7041690	treatment	flavopiridol (1 microM)	-1 hr	0 hr	1 hr
	vehicle	Mock infection	-5760 min	-5670 min	90 min
SRR8451931	vehicle	DMSO (0.1%)	-1 hr	0 hr	1 hr
	treatment	HCMV strain Towne BAC	-5760 min	-5670 min	90 min
SRR8451932	treatment	flavopiridol (1 microM)	-1 hr	0 hr	1 hr
	treatment	HCMV strain Towne BAC	-5760 min	-5670 min	90 min
SRR8452660	treatment	flavopiridol (1 microM)	-1 hr	0 hr	1 hr
	treatment	HCMV strain Towne varS	-5760 min	-5670 min	90 min
SRR8452661	vehicle	DMSO (0.1%)	-1 hr	0 hr	1 hr
	treatment	HCMV strain Towne varS	-5760 min	-5670 min	90 min
SRR8457216	treatment	flavopiridol (1 microM)	-1 hr	0 hr	1 hr
	treatment	HCMV strain TB40/E (MOI 3)	-4 hr	0 hr	4 hr
SRR8457217	vehicle	DMSO (0.1%)	-1 hr	0 hr	1 hr
	treatment	HCMV strain TB40/E (MOI 3)	-4 hr	0 hr	4 hr

Sample metrics

Sample name	Raw read length	Trim read depth	Duplication proportion	Proportion reads mapped	Exon/intron ratio	Unique read proportion	Sample QC score	Sample NRO score
SRR8456237	151	52,696,735	0.58138	0.1075	6.64505	0.30493	4	3
SRR8456238	151	45,809,327	0.61084	0.0816	9.811	0.21128	5	5
SRR8463311	151	56,865,683	0.8447	0.3997	7.15538	0.27454	4	4
SRR8463312	151	48,579,747	0.82456	0.3044	9.9172	0.20128	4	5
SRR7041686	151	63,933,220	0.51222	0.7804	2.40458	0.52697	2	1
SRR7041689	151	29,026,226	0.23401	0.445	2.08704	0.75465	2	1
SRR7041690	151	34,638,184	0.42382	0.3749	2.89201	0.50523	2	1
SRR8451931	151	28,131,938	0.57284	0.4631	2.99598	0.36597	2	1
SRR8451932	151	27,133,830	0.60316	0.4143	3.97455	0.24342	3	2
SRR8452660	151	26,184,279	0.35968	0.2181	2.79758	0.45508	4	1
SRR8452661	151	23,890,943	0.25556	0.2509	2.22244	0.63948	4	1
SRR8457216	151	63,101,686	0.59254	0.7007	3.17704	0.38214	2	2
SRR8457217	151	67,369,045	0.47125	0.7116	2.57419	0.53976	1	1

Bidirectionals

Sample name	Tfit total bidirs	Tfit promoter bidirs	Tfit intronic bidirs	Tfit intergenic bidirs	dREG total bidirs	dREG promoter bidirs	dREG intronic bidirs	dREG intergenic bidirs
SRR8456237	19613	11806	6887	1622	11124	8107	2947	784
SRR8456238	11208	7246	4089	573	7021	5723	1727	320
SRR8463311	24859	12606	9407	3508	15837	9270	5413	1962
SRR8463312	12580	7268	5100	941	10057	7271	3243	625
SRR7041686	27409	12935	9718	4780	20152	8654	8020	3528
SRR7041689	18314	8756	7386	2204	8323	5477	2594	730
SRR7041690	12313	6679	4991	987	7093	5050	2212	503
SRR8451931	12801	6770	4714	971	3011	2039	750	354
SRR8451932	11155	6848	4071	671	5654	4499	1290	365
SRR8452660	9057	5479	3308	550	3754	3035	798	277
SRR8452661	12597	6588	4618	1155	2874	2140	646	255
SRR8457216	15175	7282	6455	1363	16905	9161	6633	1807
SRR8457217	23872	10752	9051	3821	17517	7924	6793	2854